Protein Database, Human Retinal Pigment Epithelium
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چکیده
منابع مشابه
Protein database, human retinal pigment epithelium.
The retinal pigment epithelium (RPE) is a single cell layer adjacent to the rod and cone photoreceptors that plays key roles in retinal physiology and the biochemistry of vision. RPE cells were isolated from normal adult human donor eyes, subcellular fractions were prepared, and proteins were fractionated by electrophoresis. Following in-gel proteolysis, proteins were identified by peptide sequ...
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Adult human retinal pigment epithelium has been enzymatically isolated and established In tissue culture. Primary cultures survived three to six months. Occasionally, cell lines spontaneously evolved from primary cultures. Melanin granules decreased with the age of the culture, became less pigmented but retained some characterstics of melanin-producing cells. Lipofuscin granules are present, bu...
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Problems in ion and fluid transfer across the retinal pigment epithelium (RPE) are a probable cause of inappropriate accumulations of fluid between the photoreceptors of the retina and the RPE. The activities of Cl- transporters involved in basal fluid transfer across the RPE have been compared to determine whether Ca2+- or cAMP-dependent channels may be responsible for basal housekeeping level...
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Objective(s):The aim of this study was to evaluate distribution and changes of glycoconjugates of retinal photoreceptors during both pre- and postnatal development. Materials and Methods: Tissue sections from days 15 to 20 of Wistar rat embryos and 1 to 12 postnatal days of rat newborns including developing eye were prepared for lectinhistochemistry technique. Horseradish peroxidase (HRP)-label...
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Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using allografts, we established in vivo immune rejection models with induced pluri...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2003
ISSN: 1535-9476
DOI: 10.1074/mcp.d200001-mcp200